Antibacterial plastic surfaces by PVD sputter deposition

Gerald Zauner, Thorsten Schulte, Daniel Heim, Wolfgang Stadlbauer, Reinhard Mayr, Sven Wohlfarth, Paul Klemencic, Thomas Mitterlehner, Gabriele Ettenberger-Bornberg

Research output: Chapter in Book/Report/Conference proceedingsConference contribution

Abstract

This work presents first results of a research project which aims to establish plasma surface treatment methods (PVD-HF sputtering) for the antibacterial functionalization of plastic surfaces. Sputter processes are used to deposit different thin metal/metal-oxide films on to the surface of a wide range of different polymers. The according biocidal effect is evaluated afterwards by combined microbiological analysis and fluorescence microscopy. In contrast to more traditional microbial inhibition methods (like addition of metal oxide micro- particles during the extrusion process), the presented approach tries to create metal-oxide based biocidal surface properties directly at the surface by means of plasma deposition. In particular, a HF-PVD plasma sputter system (13,56 MHz) is used to deposit the following metals/oxides: Zn, Ag, Cu, etc. In this way, substrate specific characteristics of a large number of different plastics (e.g. PLA, PA6, PP, PE, HDPE, PS, ABS, PMA) are investigated. The resulting physical surface properties are characterized by atomic force microscopy (AFM), electron microscopy (EDX), scratch tests, contact angle measurements etc. Special focus is also put on the surface pre-treatment (like cleaning and activation). The coated and uncoated surfaces are then microbiologically tested, i.e. the survival rates of bacteria strains (over a defined period of time) are determined by using a fluorescence spectroscopic assay. The investigated bacteria are Escherichia coli and Pseudomonas aeruginosa, two very typical representatives of pathogenes (e.g. for nosocomial infections). The survival rates are monitored by using the LIVE/DEAD BacLight Bacterial Viability Kit (Molecular Probes) in a commercial fluorescence microscope, which allows a relatively fast estimation of a potential antimicrobial effect. Additionally, standard microbiological tests (like ATP luminescence test, Alamar-Blue method and Agar diffusion test) are conducted for more precise results.
Translated title of the contributionAntibacterial plastic surfaces by PVD sputter deposition
Original languageGerman
Title of host publication14th International Conference on Plasma Surface Engineering
Publication statusPublished - 2014
Event12th International Conference on Plasma Surface Engineering - Garmisch-Partenkirchen, Germany
Duration: 15 Sep 201419 Sep 2014

Conference

Conference12th International Conference on Plasma Surface Engineering
CountryGermany
CityGarmisch-Partenkirchen
Period15.09.201419.09.2014

Fingerprint Dive into the research topics of 'Antibacterial plastic surfaces by PVD sputter deposition'. Together they form a unique fingerprint.

Cite this