Anti-Amyloid aggregation activity of black sesame pigment: Toward a novel Alzheimer's disease preventive agent

Lucia Panzella, Thomas Eidenberger, Alessandra Napolitano

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Black sesame pigment (BSP) represents a low cost, easily accessible material of plant origin exhibiting marked antioxidant and heavy metal-binding properties with potential as a food supplement. We report herein the inhibitory properties of the potentially bioaccessible fraction of BSP following simulated gastrointestinal digestion against key enzymes involved in Alzheimer's disease (AD). HPLC analysis indicated that BSP is transformed under the pH conditions mimicking the intestinal environment and the most abundant of the released compounds was identified as vanillic acid. More than 80% inhibition of acetylcholinesterase-induced aggregation of the β-Amyloid Aβ1-40 was observed in the presence of the potentially bioaccessible fraction of BSP, which also efficiently inhibited self-induced Aβ1-42 aggregation and β-secretase (BACE-1) activity, even at high dilution. These properties open new perspectives toward the use of BSP as an ingredient of functional food or as a food supplement for the prevention of AD.

Original languageEnglish
JournalMolecules
Volume23
Issue number3
DOIs
Publication statusPublished - Mar 2018

Keywords

  • Acetylcholinesterase
  • Alzheimer's disease
  • Black sesame
  • Butyrylcholinesterase
  • Simulated digestion
  • Vanillic acid
  • β-Amyloid
  • β-secretase
  • Pigments, Biological/analysis
  • Humans
  • Peptide Fragments/chemistry
  • Chromatography, High Pressure Liquid
  • Plant Extracts/analysis
  • Sesamum/chemistry
  • Amyloid beta-Peptides/chemistry
  • Alzheimer Disease/enzymology
  • Cholinesterase Inhibitors/chemistry
  • Molecular Structure
  • Protein Aggregates/drug effects
  • Vanillic Acid/chemistry

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