A Simplified and Robust Activation Procedure of Glass Surfaces for Printing Proteins and Subcellular Micropatterning Experiments

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Abstract

Depositing biomolecule micropatterns on solid substrates via microcontact printing (µCP) usually requires complex chemical substrate modifications to initially create reactive surface groups. Here, we present a simplified activation procedure for untreated solid substrates based on a commercial polymer metal ion coating (AnteoBind™ Biosensor reagent) that allows for direct µCP and the strong attachment of proteins via avidity binding. In proof-of-concept experiments, we identified the optimum working concentrations of the surface coating, characterized the specificity of protein binding and demonstrated the suitability of this approach by subcellular micropatterning experiments in living cells. Altogether, this method represents a significant enhancement and simplification of existing µCP procedures and further increases the accessibility of protein micropatterning for cell biological research questions.

Original languageEnglish (American)
Article number140
JournalBiosensors
Volume12
Issue number3
DOIs
Publication statusPublished - 25 Feb 2022

Keywords

  • Fluorescence microscopy
  • Live cell analysis
  • Micro-contact printing
  • Micropatterning
  • Surface Properties
  • Biosensing Techniques
  • Glass/chemistry
  • Printing, Three-Dimensional
  • Polymers/chemistry

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