Abstract
The ability of bone graft substitutes to promote new bone formation has been increasingly used in the medical field to repair skeletal defects or to replace missing bone in a broad range of applications in dentistry and orthopedics. A common way to assess such materials is via micro computed tomography (µ-CT), through the density information content provided by the absorption of X-rays. Information on the chemical composition of a material can be obtained via Raman spectroscopy. By investigating a bone sample from miniature pigs containing the bone graft substitute Bio Oss ® , we pursued the target of assessing to what extent the density information gained by µ-CT imaging matches the chemical information content provided by Raman spectroscopic imaging. Raman images and Raman correlation maps of the investigated sample were used in order to generate a Raman based segmented image by means of an agglomerative, hierarchical cluster analysis. The resulting segments, showing chemically related areas, were subsequently compared with the µ-CT image by means of a one-way ANOVA. We found out that to a certain extent typical gray-level values (and the related histograms) in the µ-CT image can be reliably related to specific segments within the image resulting from the cluster analysis.
Original language | English |
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Pages (from-to) | 3831-3853 |
Number of pages | 23 |
Journal | Materials Science |
Volume | 8 |
Issue number | 7 |
DOIs | |
Publication status | Published - Jun 2015 |
Keywords
- computed tomography
- Raman spectroscopy
- bone
- Bio-Oss
- cluster analysis
- image segmentation
- Cluster analysis
- Bio-Oss®
- Image segmentation
- Bone
- X-ray micro computed tomography