Description
IntroductionBioavailability of fat-soluble vitamins is frequently limited due to insufficient intestinal absorption. Micelles as vesicles for these hydrophobic vitamins were therefore tested on increasing the vitamin’s absorption in intestinal cells and in an alternative route for absorption – the buccal mucosa. In addition, it was investigated whether the increase in vitamin bioavailability is also accompanied by increased biological effects.
Method
The fat-soluble vitamins vitamin D3 (cholecalciferol), vitamin E (alpha-tocopherol acetate) or vitamin K2 (menaquinone-7) were solubilized into micelles. Vitamins were applied to intestinal CaCo-2 or buccal TR146 cells in either micellated or non-micellated form and cellular vitamin uptake after 4 h incubation was quantitated by HPLC-MS analysis. As far as an increase in uptake by micellization was observed, further investigation was carried out on whether the increase in uptake is indeed accompanied by more pronounced biological effects. Parameters analyzed included genes known to be induced by the individual vitamins such as the calcium-selective ion channel TRPV6 (Transient Receptor Potential channel family, Vanilloid subfamily member 6) for vitamin D3.
Results
Absorption of vitamin D3, E and K2 was significantly increased in both CaCo-2 and TR146 cells when vitamins were in micellated form. Further evaluation in CaCo-2 cells showed that micellization also significantly increased biological effects such as vitamin D-induced TRPV6 gene expression.
Conclusion
Micellization not only increased absorption of fat-soluble vitamins but also increased individual functional parameters, and therefore is a novel approach to enhance bioavailability and bioefficacy of fat-soluble vitamins.
Period | 21 Sept 2023 |
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Event title | International Vitamin Conference |
Event type | Conference |
Location | Copenhagen, DenmarkShow on map |
Degree of Recognition | International |
Related content
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Research output
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Micellization increases bioavailability and bioefficacy of fat-soluble vitamins in buccal and intestinal cells
Research output: Contribution to conference › Abstract