TY - JOUR
T1 - The nucleus measures shape changes for cellular proprioception to control dynamic cell behavior
AU - Venturini, Valeria
AU - Pezzano, Fabio
AU - Català Castro, Frederic
AU - Häkkinen, Hanna Maria
AU - Jiménez-Delgado, Senda
AU - Colomer-Rosell, Mariona
AU - Marro, Monica
AU - Tolosa-Ramon, Queralt
AU - Paz-López, Sonia
AU - Valverde, Miguel A.
AU - Weghuber, Julian
AU - Loza-Alvarez, Pablo
AU - Krieg, Michael
AU - Wieser, Stefan
AU - Ruprecht, Verena
N1 - Funding Information:
V.V. acknowledges support from the ICFOstepstone PhD Programme funded by the European Union's Horizon 2020 research and innovation program under Marie Skłodowska-Curie grant agreement 665884. F.P. and Q.T.-R. acknowledge grants funded by Ministerio de Ciencia, Innovación y Universidades and Fondo Social Europeo (FSE) (BES2017-080523-SO, PRE2018-084393). M.A.V. acknowledges support from the Spanish Ministry of Science, Education and Universities through grant RTI2018-099718-B-100 and an institutional “Maria de Maeztu Programme” for Units of Excellence in R&D (CEX2018-000792-M) and FEDER funds. J.W. was supported by the Christian Doppler Forschungsgesellschaft (Josef Ressel Center for Phytogenic Drug Research). S.W. and M.K. acknowledge support from the Spanish Ministry of Economy and Competitiveness through the Severo Ochoa program for Centres of Excellence in R&D (CEX2019-000910-S), from Fundació Privada Cellex, Fundación Mig-Puig, and from Generalitat de Catalunya through the CERCA program and LaserLab (654148). M.K. acknowledges support through Spanish Ministry of Economy and Competitiveness (RYC-2015-17935, EQC2018-005048-P, AEI-010500-2018-228, and PGC2018-097882-A-I00), Generalitat de Catalunya (2017 SGR 1012), the ERC (715243), and the HFSPO (CDA00023/2018). S.W. acknowledges support through the Spanish Ministry of Economy and Competitiveness via MINECO's Plan Nacional (PGC2018-098532-A-I00). V.R. acknowledges support from the Spanish Ministry of Science and Innovation to the EMBL partnership, the Centro de Excelencia Severo Ochoa, the CERCA Programme/Generalitat de Catalunya, and the MINECO's Plan Nacional (BFU2017-86296-P)
Publisher Copyright:
© 2020 American Association for the Advancement of Science. All rights reserved.
PY - 2020/10/16
Y1 - 2020/10/16
N2 - The physical microenvironment regulates cell behavior during tissue development and homeostasis. How single cells decode information about their geometrical shape under mechanical stress and physical space constraints within tissues remains largely unknown. Here, using a zebrafish model, we show that the nucleus, the biggest cellular organelle, functions as an elastic deformation gauge that enables cells to measure cell shape deformations. Inner nuclear membrane unfolding upon nucleus stretching provides physical information on cellular shape changes and adaptively activates a calcium-dependent mechanotransduction pathway, controlling actomyosin contractility and migration plasticity. Our data support that the nucleus establishes a functional module for cellular proprioception that enables cells to sense shape variations for adapting cellular behavior to their microenvironment.
AB - The physical microenvironment regulates cell behavior during tissue development and homeostasis. How single cells decode information about their geometrical shape under mechanical stress and physical space constraints within tissues remains largely unknown. Here, using a zebrafish model, we show that the nucleus, the biggest cellular organelle, functions as an elastic deformation gauge that enables cells to measure cell shape deformations. Inner nuclear membrane unfolding upon nucleus stretching provides physical information on cellular shape changes and adaptively activates a calcium-dependent mechanotransduction pathway, controlling actomyosin contractility and migration plasticity. Our data support that the nucleus establishes a functional module for cellular proprioception that enables cells to sense shape variations for adapting cellular behavior to their microenvironment.
KW - Actomyosin/metabolism
KW - Animals
KW - Cell Movement
KW - Cell Shape
KW - Lipase/metabolism
KW - Mechanotransduction, Cellular
KW - Myosin Type II/metabolism
KW - Nuclear Envelope/physiology
KW - Phospholipases A2, Cytosolic/metabolism
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=85093495815&partnerID=8YFLogxK
U2 - 10.1126/science.aba2644
DO - 10.1126/science.aba2644
M3 - Article
C2 - 33060331
AN - SCOPUS:85093495815
SN - 0036-8075
VL - 370
JO - Science (New York, N.Y.)
JF - Science (New York, N.Y.)
IS - 6514
M1 - 2644
ER -