@article{1012813030d24d03aa128d9d26705603,
title = "T cell activation is determined by the number of presented antigens.",
abstract = "Antigen recognition is a key event during T cell activation. Here, we introduce nanopatterned antigen arrays that mimic the antigen presenting cell surface during T cell activation. The assessment of activation related events revealed the requirement of a minimal density of 90-140 stimulating major histocompatibility complex class II proteins (pMHC) molecules per μm 2. We demonstrate that these substrates induce T cell responses in a pMHC dose-dependent manner and that the number of presented pMHCs dominates over local pMHC density.",
keywords = "Nanopattern, T cell activation, TCR microclusters, artificial antigen-presenting cell, immunological synapse, nanostructures, Humans, Synapses/immunology, Major Histocompatibility Complex/immunology, Membrane Proteins/immunology, Lymphocyte Activation/immunology, Nanoparticles/chemistry, Antigen-Presenting Cells/chemistry, T-Lymphocytes/chemistry",
author = "J Deeg and M Axmann and J Matic and A Liapis and D Depoil and J Afrose and S Curado and ML Dustin and JP Spatz",
note = "Copyright: Copyright 2013 Elsevier B.V., All rights reserved.",
year = "2013",
month = nov,
day = "13",
doi = "10.1021/nl403266t",
language = "English",
volume = "13",
pages = "5619--5626",
journal = "Nano Letters",
issn = "1530-6984",
publisher = "American Chemical Society",
number = "11",
}