Real-time 3D single-molecule localization microscopy analysis using lookup tables

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

2 Zitate (Scopus)
11 Downloads (Pure)

Abstract

Herein, we present a new algorithm for real-time analysis of 3D single molecule localization microscopy images with a small impact on fitting accuracy using lookup-tables with discrete xyz-positions. The algorithm realizes real-time visualization during acquisition. We demonstrate its performance on simulated and measured data. Additionally, combining real-time fitting with a feedback loop controlling the activation laser pulse keeps the number of emitters per image frame constant.
OriginalspracheEnglisch
Seiten (von - bis)4955-4968
Seitenumfang14
FachzeitschriftBiomedical Optics Express
Jahrgang12
Ausgabenummer8
DOIs
PublikationsstatusVeröffentlicht - 1 Aug. 2021

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