Real-time 3D single-molecule localization microscopy analysis using lookup tables

Fabian Hauser; Jaroslaw Jacak

doi:10.1364/boe.424016

Real-time 3D single-molecule localization microscopy analysis using lookup tables

Fabian Hauser, Jaroslaw Jacak

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

3 Zitate (Scopus)

51 Downloads (Pure)

Abstract

Herein, we present a new algorithm for real-time analysis of 3D single molecule localization microscopy images with a small impact on fitting accuracy using lookup-tables with discrete xyz-positions. The algorithm realizes real-time visualization during acquisition. We demonstrate its performance on simulated and measured data. Additionally, combining real-time fitting with a feedback loop controlling the activation laser pulse keeps the number of emitters per image frame constant.

Originalsprache	Englisch
Seiten (von - bis)	4955-4968
Seitenumfang	14
Fachzeitschrift	Biomedical Optics Express
Jahrgang	12
Ausgabenummer	8
DOIs	https://doi.org/10.1364/boe.424016
Publikationsstatus	Veröffentlicht - 1 Aug. 2021

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10.1364/boe.424016Lizenz: CC BY

boe-12-8-4955Endgültige, publizierte Fassung, 8,03 MBLizenz: CC BY

https://www.osapublishing.org/boe/fulltext.cfm?uri=boe-12-8-4955Lizenz: CC BY

Andere Dateien und Links

Link zur Publikation in Scopus

Vascular MicroLab
Jacak, J. (Leitende(r) Forscher/-in) & Hauser, F. (Weitere Forschende)
Dissertationsprogramm der Fachhochschule OÖ
12.11.2018 → 11.11.2021
Projekt: Forschungsprojekt

3D localization microscopy of activated platelets on injured cells within a microfluidics
Hauser, F. (Redner*in)
14 Sep. 2021
Aktivität: Gespräch oder Vortrag › Vortrag
3D localization microscopy of human platelet activation via interaction with injured cells
Hauser, F. (Redner*in)
2 Sep. 2021
Aktivität: Gespräch oder Vortrag › Vortrag

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Real-time 3D single-molecule localization microscopy analysis using lookup tables

Abstract

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Andere Dateien und Links

Fingerprint

Projekte

Vascular MicroLab

Aktivitäten

3D localization microscopy of activated platelets on injured cells within a microfluidics

3D localization microscopy of human platelet activation via interaction with injured cells

Zitieren