Protein micropatterning assay: Quantitative analysis of protein–protein interactions

Gerhard Schütz, Julian Weghuber, Peter Lanzerstorfer, Eva Sevcsik

Publikation: Beitrag in Buch/Bericht/TagungsbandKapitelBegutachtung

11 Zitate (Scopus)

Abstract

Characterization, especially quantification, of protein interactions in live cells is usually not an easy endeavor. Here, we describe a straightforward method to identify and quantify the interaction of a membrane protein (“bait”) and a fluorescently labeled interaction partner (“prey”) (membrane-bound or cyto-solic) in live cells using Total Internal Reflection Fluorescence microscopy. The bait protein is immobilized within patterns in the plasma membrane (e.g., via an antibody); the bait–prey interaction strength can be quantified by determining the prey bulk fluorescence intensity with respect to the bait patterns. This method is particularly suitable also for the analysis of weak, transient interactions that are not easily accessible with other methods.

OriginalspracheEnglisch
TitelMethods in Molecular Biology
Herausgeber (Verlag)Humana Press Inc.
Seiten261-270
Seitenumfang10
Band1550
DOIs
PublikationsstatusVeröffentlicht - 2017

Publikationsreihe

NameMethods in Molecular Biology
Band1550
ISSN (Print)1064-3745

Fingerprint

Untersuchen Sie die Forschungsthemen von „Protein micropatterning assay: Quantitative analysis of protein–protein interactions“. Zusammen bilden sie einen einzigartigen Fingerprint.

Zitieren