High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler

Taras Sych; Jan Schlegel; Hanna M.G. Barriga; Miina Ojansivu; Leo Hanke; Florian Weber; R. Beklem Bostancioglu; Kariem Ezzat; Herbert Stangl; Birgit Plochberger; Jurga Laurencikiene; Samir El Andaloussi; Daniel Fürth; Molly M. Stevens; Erdinc Sezgin

doi:10.1038/s41587-023-01825-5

High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler

Taras Sych
, Jan Schlegel
, Hanna M.G. Barriga
, Miina Ojansivu
, Leo Hanke
, Florian Weber
, R. Beklem Bostancioglu
, Kariem Ezzat
, Herbert Stangl
, Birgit Plochberger
, Jurga Laurencikiene
, Samir El Andaloussi
, Daniel Fürth
, Molly M. Stevens
, Erdinc Sezgin^*

^*Korrespondierende/r Autor/-in für diese Arbeit

Research Center Linz

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

44 Zitate (Scopus)

Abstract

We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5–200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.

Originalsprache	Englisch
Seiten (von - bis)	587-590
Seitenumfang	4
Fachzeitschrift	Nature Biotechnology
Jahrgang	42
Ausgabenummer	4
Frühes Online-Datum	Juni 2023
DOIs	https://doi.org/10.1038/s41587-023-01825-5
Publikationsstatus	Veröffentlicht - Apr. 2024

Zugriff auf Dokument

10.1038/s41587-023-01825-5

Andere Dateien und Links

Link zur Publikation in Scopus

Zitieren

Sych, T., Schlegel, J., Barriga, H. M. G., Ojansivu, M., Hanke, L., Weber, F., Beklem Bostancioglu, R., Ezzat, K., Stangl, H., Plochberger, B., Laurencikiene, J., El Andaloussi, S., Fürth, D., Stevens, M. M., & Sezgin, E. (2024). High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler. Nature Biotechnology, 42(4), 587-590. https://doi.org/10.1038/s41587-023-01825-5

@article{587c95cc87dc4c5c88f2d12d7b7b9b11,

title = "High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler",

abstract = "We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5–200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.",

keywords = "Particle Size, Liposomes/chemistry, Nanoparticles/chemistry",

author = "Taras Sych and Jan Schlegel and Barriga, \{Hanna M.G.\} and Miina Ojansivu and Leo Hanke and Florian Weber and \{Beklem Bostancioglu\}, R. and Kariem Ezzat and Herbert Stangl and Birgit Plochberger and Jurga Laurencikiene and \{El Andaloussi\}, Samir and Daniel F{\"u}rth and Stevens, \{Molly M.\} and Erdinc Sezgin",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2023.",

year = "2024",

month = apr,

doi = "10.1038/s41587-023-01825-5",

language = "English",

volume = "42",

pages = "587--590",

journal = "Nature Biotechnology",

issn = "1087-0156",

publisher = "Nature Research",

number = "4",

}

Sych, T, Schlegel, J, Barriga, HMG, Ojansivu, M, Hanke, L, Weber, F, Beklem Bostancioglu, R, Ezzat, K, Stangl, H, Plochberger, B, Laurencikiene, J, El Andaloussi, S, Fürth, D, Stevens, MM & Sezgin, E 2024, 'High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler', Nature Biotechnology, Jg. 42, Nr. 4, S. 587-590. https://doi.org/10.1038/s41587-023-01825-5

TY - JOUR

T1 - High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler

AU - Sych, Taras

AU - Schlegel, Jan

AU - Barriga, Hanna M.G.

AU - Ojansivu, Miina

AU - Hanke, Leo

AU - Weber, Florian

AU - Beklem Bostancioglu, R.

AU - Ezzat, Kariem

AU - Stangl, Herbert

AU - Plochberger, Birgit

AU - Laurencikiene, Jurga

AU - El Andaloussi, Samir

AU - Fürth, Daniel

AU - Stevens, Molly M.

AU - Sezgin, Erdinc

PY - 2024/4

Y1 - 2024/4

N2 - We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5–200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.

AB - We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5–200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.

KW - Particle Size

KW - Liposomes/chemistry

KW - Nanoparticles/chemistry

UR - https://www.scopus.com/pages/publications/85163136152

U2 - 10.1038/s41587-023-01825-5

DO - 10.1038/s41587-023-01825-5

M3 - Article

C2 - 37308687

AN - SCOPUS:85163136152

SN - 1087-0156

VL - 42

SP - 587

EP - 590

JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 4

ER -

High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler

Abstract

Zugriff auf Dokument

Andere Dateien und Links

Fingerprint

Zitieren