TY - JOUR
T1 - High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler
AU - Sych, Taras
AU - Schlegel, Jan
AU - Barriga, Hanna M.G.
AU - Ojansivu, Miina
AU - Hanke, Leo
AU - Weber, Florian
AU - Beklem Bostancioglu, R.
AU - Ezzat, Kariem
AU - Stangl, Herbert
AU - Plochberger, Birgit
AU - Laurencikiene, Jurga
AU - El Andaloussi, Samir
AU - Fürth, Daniel
AU - Stevens, Molly M.
AU - Sezgin, Erdinc
N1 - Publisher Copyright:
© The Author(s) 2023.
PY - 2024/4
Y1 - 2024/4
N2 - We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5–200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.
AB - We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5–200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.
KW - Particle Size
KW - Liposomes/chemistry
KW - Nanoparticles/chemistry
UR - http://www.scopus.com/inward/record.url?scp=85163136152&partnerID=8YFLogxK
U2 - 10.1038/s41587-023-01825-5
DO - 10.1038/s41587-023-01825-5
M3 - Article
C2 - 37308687
AN - SCOPUS:85163136152
SN - 1087-0156
VL - 42
SP - 587
EP - 590
JO - Nature Biotechnology
JF - Nature Biotechnology
IS - 4
ER -