TY - JOUR
T1 - Gold Nanoislands Grown on Multiphoton Polymerized Structures as Substrate for Enzymatic Reactions
AU - Buchegger, Bianca
AU - Vidal, Cynthia
AU - Neuwirth, Julia
AU - Buchroithner, Boris
AU - Karner, Andreas
AU - Hochreiner, Armin
AU - Klar, Thomas
AU - Jacak, Jaroslaw
N1 - Funding Information:
We would like to thank Bernhard Fragner and Alfred Nimmervoll for technical support and Heidi Piglmayer-Brezina for the EDX and SEM measurements. This work was funded by the Basic Funding of the Upper Austrian University of Applied Sciences Project—PolFunk and the TiMED funding of the Upper Austrian University of Applied Sciences TC-LOEM, the Austria Science Fund (FWF) project P 31827-B21, by the European Fund for Regional Development (EFRE, IWB2020), and by the Federal State of Upper Austria.
Publisher Copyright:
Copyright © 2019 American Chemical Society.
PY - 2019/10/7
Y1 - 2019/10/7
N2 - We show that multiphoton thiol–ene polymerized structures comprise unreacted thiol moieties, which can be used for postpolymerization gold metallization. Some of the thiol groups located at the surface are not involved in thiol–ene reactions and, therefore, can serve as nucleation seeds for the synthesis of ∼50 nm sized gold nanoislands. Additionally, we show that the nanoislands can be used for immobilization of fluorescent molecules. We observed a significant enhancement of the fluorescence signal on the nanoisland-functionalized polymer structures when compared to the structured polymers without gold. To show a possible application, we bound peroxidase to the gold nanoislands. Peroxidase activity has been verified by chemiluminescence of the converted luminol substrate.
AB - We show that multiphoton thiol–ene polymerized structures comprise unreacted thiol moieties, which can be used for postpolymerization gold metallization. Some of the thiol groups located at the surface are not involved in thiol–ene reactions and, therefore, can serve as nucleation seeds for the synthesis of ∼50 nm sized gold nanoislands. Additionally, we show that the nanoislands can be used for immobilization of fluorescent molecules. We observed a significant enhancement of the fluorescence signal on the nanoisland-functionalized polymer structures when compared to the structured polymers without gold. To show a possible application, we bound peroxidase to the gold nanoislands. Peroxidase activity has been verified by chemiluminescence of the converted luminol substrate.
UR - http://www.scopus.com/inward/record.url?scp=85083734086&partnerID=8YFLogxK
U2 - 10.1021/acsmaterialslett.9b00182
DO - 10.1021/acsmaterialslett.9b00182
M3 - Article
SN - 2639-4979
VL - 1
SP - 399
EP - 403
JO - ACS Materials Letters
JF - ACS Materials Letters
IS - 4
ER -