Frequency-domain photoacoustic and fluorescence microscopy: Application on labeled and unlabeled cells

Gregor Langer, Bianca Buchegger, Jaroslaw Jacak, Karoline Pfeffer, Sven Wohlfarth, Günther Hannesschläger, Thomas A. Klar, Thomas Berer

Publikation: Beitrag in Buch/Bericht/TagungsbandKonferenzbeitragBegutachtung

2 Zitate (Scopus)

Abstract

In this paper, multimodal optical-resolution frequency-domain photoacoustic and fluorescence scanning microscopy is presented on labeled and unlabeled cells. In many molecules, excited electrons relax radiatively and non-radiatively, leading to fluorescence and photoacoustic signals, respectively. Both signals can then be detected simultaneously. There also exist molecules, e.g. hemoglobin, which do not exhibit fluorescence, but provide photoacoustic signals solely. Other molecules, especially fluorescent dyes, preferentially exhibit fluorescence. The fluorescence quantum yield of a molecule and with it the strength of photoacoustic and fluorescence signals depends on the local environment, e.g. on the pH. Therefore, the local distribution of the simultaneously recorded photoacoustic and fluorescence signals may be used in order to obtain information about the local chemistry.

OriginalspracheEnglisch
TitelMultimodal Biomedical Imaging XIII
Redakteure/-innenFred S. Azar, Xavier Intes
Herausgeber (Verlag)SPIE
ISBN (elektronisch)9781510614598
DOIs
PublikationsstatusVeröffentlicht - 2018
VeranstaltungMultimodal Biomedical Imaging XIII 2018 - San Francisco, USA/Vereinigte Staaten
Dauer: 27 Jän. 201828 Jän. 2018

Publikationsreihe

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Band10487
ISSN (Print)1605-7422

Konferenz

KonferenzMultimodal Biomedical Imaging XIII 2018
Land/GebietUSA/Vereinigte Staaten
OrtSan Francisco
Zeitraum27.01.201828.01.2018

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