Fabrication of Highly Ordered Gold Nanoparticle Arrays Templated by Crystalline Lattices of Bacterial S-Layer Protein

(Figure Presented) Biotemplating: The square lattice of S-layer protein rSbpA-Cys is used as a biotemplate for directing self-assembly of gold nanoparticle (NP) arrays with controlled distance (see graphic). The gold NPs are specifically bound to the exposed cysteines on the S-layer protein lattice and assembled into square symmetry nanoarrays, which show the same periodicity as the S-layer template.