TY - JOUR
T1 - Dimerisation of the Yeast K+ Translocation Protein Trk1 Depends on the K+ Concentration
AU - Kulik, Natalia
AU - Kale, Deepika
AU - Spurna, Karin
AU - Shamayeva, Katsiaryna
AU - Hauser, Fabian
AU - Milic, Sandra
AU - Janout, Hannah
AU - Zayats, Vasilina
AU - Jacak, Jaroslaw
AU - Ludwig, Jost
N1 - Publisher Copyright:
© 2022 by the authors.
PY - 2023/1
Y1 - 2023/1
N2 - In baker’s yeast (Saccharomyces cerevisiae), Trk1, a member of the superfamily of K-transporters (SKT), is the main K+ uptake system under conditions when its concentration in the environment is low. Structurally, Trk1 is made up of four domains, each similar and homologous to a K-channel α subunit. Because most K-channels are proteins containing four channel-building α subunits, Trk1 could be functional as a monomer. However, related SKT proteins TrkH and KtrB were crystallised as dimers, and for Trk1, a tetrameric arrangement has been proposed based on molecular modelling. Here, based on Bimolecular Fluorescence Complementation experiments and single-molecule fluorescence microscopy combined with molecular modelling; we provide evidence that Trk1 can exist in the yeast plasma membrane as a monomer as well as a dimer. The association of monomers to dimers is regulated by the K+ concentration.
AB - In baker’s yeast (Saccharomyces cerevisiae), Trk1, a member of the superfamily of K-transporters (SKT), is the main K+ uptake system under conditions when its concentration in the environment is low. Structurally, Trk1 is made up of four domains, each similar and homologous to a K-channel α subunit. Because most K-channels are proteins containing four channel-building α subunits, Trk1 could be functional as a monomer. However, related SKT proteins TrkH and KtrB were crystallised as dimers, and for Trk1, a tetrameric arrangement has been proposed based on molecular modelling. Here, based on Bimolecular Fluorescence Complementation experiments and single-molecule fluorescence microscopy combined with molecular modelling; we provide evidence that Trk1 can exist in the yeast plasma membrane as a monomer as well as a dimer. The association of monomers to dimers is regulated by the K+ concentration.
KW - bimolecular fluorescence complementation
KW - dimerisation
KW - K translocation
KW - MD simulation
KW - molecular modelling
KW - Saccharomyces cerevisiae
KW - Translocation, Genetic
KW - Potassium/metabolism
KW - Fungal Proteins/metabolism
KW - Cation Transport Proteins/genetics
KW - Saccharomyces cerevisiae/metabolism
KW - Biological Transport
KW - Cell Membrane/metabolism
KW - Saccharomyces cerevisiae Proteins/genetics
KW - Carrier Proteins/metabolism
UR - http://www.scopus.com/inward/record.url?scp=85145987316&partnerID=8YFLogxK
U2 - 10.3390/ijms24010398
DO - 10.3390/ijms24010398
M3 - Article
C2 - 36613841
AN - SCOPUS:85145987316
SN - 1661-6596
VL - 24
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 1
M1 - 398
ER -