Dimerisation of the Yeast K+ Translocation Protein Trk1 Depends on the K+ Concentration

Natalia Kulik, Deepika Kale, Karin Spurna, Katsiaryna Shamayeva, Fabian Hauser, Sandra Milic, Hannah Janout, Vasilina Zayats, Jaroslaw Jacak, Jost Ludwig

Publikation: Beitrag in FachzeitschriftArtikelBegutachtung

Abstract

In baker’s yeast (Saccharomyces cerevisiae), Trk1, a member of the superfamily of K-transporters (SKT), is the main K+ uptake system under conditions when its concentration in the environment is low. Structurally, Trk1 is made up of four domains, each similar and homologous to a K-channel α subunit. Because most K-channels are proteins containing four channel-building α subunits, Trk1 could be functional as a monomer. However, related SKT proteins TrkH and KtrB were crystallised as dimers, and for Trk1, a tetrameric arrangement has been proposed based on molecular modelling. Here, based on Bimolecular Fluorescence Complementation experiments and single-molecule fluorescence microscopy combined with molecular modelling; we provide evidence that Trk1 can exist in the yeast plasma membrane as a monomer as well as a dimer. The association of monomers to dimers is regulated by the K+ concentration.

OriginalspracheEnglisch
Aufsatznummer398
FachzeitschriftInternational Journal of Molecular Sciences
Jahrgang24
Ausgabenummer1
DOIs
PublikationsstatusVeröffentlicht - Jän. 2023

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