@article{cdc0795f04e54fddb64964d8dad48ff1,
title = "Determination of interaction kinetics between the T cell receptor and peptide-loaded MHC class II via single-molecule diffusion measurements.",
abstract = "The binding of peptide-loaded major histocompatibility complex (pMHC) to the T cell receptor (TCR) represents the central step in T cell antigen recognition. It proceeds in the cell contact area between a T cell and an antigen-presenting cell termed the immunological synapse. An important and unresolved issue is how T cells discriminate between potentially harmful and harmless antigens. One limitation has been the difficulty to measure interaction parameters directly, that is, as they occur in the immunological synapse. Here we present a single-molecule approach to determine pMHC-TCR interaction kinetics in situ based on diffusion analysis of dye-labeled pMHC. We find synaptic off-rates >10-fold accelerated when compared to the dissociation of purified proteins measured in vitro.",
keywords = "Biophysics/methods, Diffusion, Histocompatibility Antigens Class II/immunology, Humans, Kinetics, Models, Immunological, Peptides/immunology, Protein Binding/immunology, Receptors, Antigen, T-Cell/immunology, Time Factors",
author = "M Axmann and JB Huppa and MM Davis and GJ Sch{\"u}tz",
note = "Funding Information: This work was supported by the GEN-AU project of the Austrian Federal Ministry for Science and Research and the Austrian Science Fund (FWF project Y250-B10) to G.J.S.; M.A. was supported by the Max Planck Society and the Schr{\"o}dinger Fellowship program (project. J3086). M.M.D. was supported by grants from the National Institutes of Health (RO1 AI52211) and the Howard Hughes Medical Institute; J.B.H. was partially supported by the Immunology Frontier Research Center consortium, the National Institutes of Health (RO1 AI52211), and the Howard Hughes Medical Institute. Copyright: Copyright 2013 Elsevier B.V., All rights reserved.",
year = "2012",
month = jul,
day = "18",
doi = "10.1016/j.bpj.2012.06.019",
language = "English",
volume = "103",
pages = "L17--L19",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Elsevier",
number = "2",
}