@inproceedings{1c9e65a10df348cebcf251f5dedf361b,
title = "Combining TIR and FRET: From fluorescence microscopy to a multi-well reader system",
abstract = "In view of a pharmacological test system with living cells we examined F{\"o}rster Resonance Energy Transfer (FRET) between fluorescent proteins upon Total Internal Reflection (TIR) of a laser beam. We measured in particular HeLa cells expressing membrane associated EGFR-CFP and Grb2-YFP prior to and subsequent to stimulation with the epidermal growth factor EGF. In addition, HeLa cells expressing a membrane associated Green Fluorescent Protein (HeLa hFR-GPI-GFP) were incubated with the energy acceptor Nile Red for further validation. Measurements range from fluorescence microscopy to Fluorescence Lifetime Imaging (FLIM) of a larger number of samples in a TIR reader.",
keywords = "FLIM, Fluorescence microscopy, Fluorescence reader, FRET, Living cells, TIR",
author = "Herbert Schneckenburger and Petra Weber and Michael Wagner and Sandra Enderle and Julian Weghuber and Peter Lanzerstorfer",
year = "2019",
doi = "10.1117/12.2526416",
language = "English",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Emmanuel Beaurepaire and Pavone, \{Francesco Saverio\}",
booktitle = "Advances in Microscopic Imaging II",
address = "United States",
note = "Advances in Microscopic Imaging II 2019 ; Conference date: 26-06-2019 Through 27-06-2019",
}