Co-localization of CD3 and prion protein in Jurkat lymphocytes after hypothermal stimulation

Susanne Wurm; Christian Paar; Alois Sonnleitner; Max Sonnleitner; Otmar Höglinger; Christoph Romanin; Christian Wechselberger

doi:10.1016/j.febslet.2004.03.114

Co-localization of CD3 and prion protein in Jurkat lymphocytes after hypothermal stimulation

Susanne Wurm, Christian Paar, Alois Sonnleitner, Max Sonnleitner, Otmar Höglinger, Christoph Romanin, Christian Wechselberger

Publikation: Beitrag in Fachzeitschrift › Artikel › Begutachtung

13 Zitate (Scopus)

Abstract

While long-term effects of temperature treatment in respect of, e.g., gene-expression and cellular function have already been studied in some detail, nothing is known on the physiological responses of lymphocytes during short-term hypothermal shifts. In this report, we characterized the effects of such a stimulation using the human lymphocyte cell line Jurkat E6.1 and present evidence that warming from 4 to 37 degrees C for only 2 min is sufficient to cause co-localization of CD3, prion protein and the lipid-raft ganglioside GM1 paralleling lymphocyte activation as observed by Ca(2+) mobilization and mitogen-activated protein kinase-phosphorylation.

Originalsprache	Englisch
Seiten (von - bis)	121-125
Seitenumfang	5
Fachzeitschrift	FEBS Letters
Jahrgang	566
Ausgabenummer	1-3
DOIs	https://doi.org/10.1016/j.febslet.2004.03.114
Publikationsstatus	Veröffentlicht - 21 Mai 2004
Extern publiziert	Ja

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title = "Co-localization of CD3 and prion protein in Jurkat lymphocytes after hypothermal stimulation",

abstract = "While long-term effects of temperature treatment in respect of, e.g., gene-expression and cellular function have already been studied in some detail, nothing is known on the physiological responses of lymphocytes during short-term hypothermal shifts. In this report, we characterized the effects of such a stimulation using the human lymphocyte cell line Jurkat E6.1 and present evidence that warming from 4 to 37 degrees C for only 2 min is sufficient to cause co-localization of CD3, prion protein and the lipid-raft ganglioside GM1 paralleling lymphocyte activation as observed by Ca(2+) mobilization and mitogen-activated protein kinase-phosphorylation.",

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AU - Wurm, Susanne

AU - Paar, Christian

AU - Sonnleitner, Alois

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AU - Höglinger, Otmar

AU - Romanin, Christoph

AU - Wechselberger, Christian

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AB - While long-term effects of temperature treatment in respect of, e.g., gene-expression and cellular function have already been studied in some detail, nothing is known on the physiological responses of lymphocytes during short-term hypothermal shifts. In this report, we characterized the effects of such a stimulation using the human lymphocyte cell line Jurkat E6.1 and present evidence that warming from 4 to 37 degrees C for only 2 min is sufficient to cause co-localization of CD3, prion protein and the lipid-raft ganglioside GM1 paralleling lymphocyte activation as observed by Ca(2+) mobilization and mitogen-activated protein kinase-phosphorylation.

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KW - TCR, T-cell receptor

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KW - T-Lymphocytes/metabolism

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KW - Cytochalasin D/pharmacology

KW - beta-Cyclodextrins

KW - Cell Membrane/metabolism

KW - Mitogen-Activated Protein Kinases/metabolism

KW - Prions/metabolism

KW - Fluorescent Antibody Technique

KW - Hypothermia, Induced

KW - Enzyme Activation

KW - Cholesterol/deficiency

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Co-localization of CD3 and prion protein in Jurkat lymphocytes after hypothermal stimulation

Abstract

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