TY - CHAP
T1 - Atomic force microscopy
AU - Duman, M.
AU - Neundlinger, I.
AU - Zhu, R.
AU - Preiner, J.
AU - Lamprecht, C.
AU - Chtcheglova, L. A.
AU - Rankl, C.
AU - Puntheeranurak, T.
AU - Ebner, A.
AU - Hinterdorfer, P.
PY - 2012
Y1 - 2012
N2 - Atomic force microscopy (AFM) allows high-resolution topographical images to be obtained at the nanometer scale together with single molecule force measurements on biological samples in their physiological environment. Therefore, it is a powerful tool for detecting the forces and the dynamics of the interaction between individual ligands and receptors on cellular surfaces. This article describes sample preparation methods, different AFM imaging modes, AFM tip functionalization, current methodology, and applications of molecular recognition studies using AFM.
AB - Atomic force microscopy (AFM) allows high-resolution topographical images to be obtained at the nanometer scale together with single molecule force measurements on biological samples in their physiological environment. Therefore, it is a powerful tool for detecting the forces and the dynamics of the interaction between individual ligands and receptors on cellular surfaces. This article describes sample preparation methods, different AFM imaging modes, AFM tip functionalization, current methodology, and applications of molecular recognition studies using AFM.
KW - Atomic force microscopy
KW - Cell fixation
KW - Cell immobilization
KW - Cellular membrane
KW - Contact mode imaging
KW - Dynamic mode imaging
KW - Fluorescence microscopy
KW - Force mapping
KW - Intermittent (tapping) mode imaging
KW - Magnetic AC mode imaging
KW - Membrane channels
KW - Membrane receptors
KW - Molecular recognition force spectroscopy
KW - Simultaneous topography and recognition imaging
UR - https://www.scopus.com/pages/publications/84882787867
U2 - 10.1016/B978-0-12-374920-8.00213-7
DO - 10.1016/B978-0-12-374920-8.00213-7
M3 - Chapter
AN - SCOPUS:84882787867
SN - 9780080957180
VL - 2
SP - 111
EP - 143
BT - Comprehensive Biophysics
PB - Elsevier
ER -