Abstract

Extracellular vesicles (EV) enable cell-to-cell communication in the body of an organism and carry significant potential in the medical field as disease indicators, tissue regeneration, and drug carriers. We developed two workflows to analyze EV data based on microscopy images reliably. The first workflow enables determining the total number of fluorophores per EV by analyzing the photobleaching step counts in a stepwise photobleaching experiment recorded through fluorescence microscopy. Furthermore, we present a workflow for the quality assessment of EV populations through multimodal imaging. Thus, enabling quantification of the purification quality (differentiation between EVs and other components) and the labeling ratio. Both workflows have shown excellent results on various data sets and under various conditions.

OriginalspracheEnglisch
TitelBiomedical Engineering Systems and Technologies - 15th International Joint Conference, BIOSTEC 2022, Revised Selected Papers
Redakteure/-innenAna Cecília A. Roque, Denis Gracanin, Ronny Lorenz, Athanasios Tsanas, Nathalie Bier, Ana Fred, Hugo Gamboa
Herausgeber (Verlag)Springer
Kapitel2
Seiten16-33
Seitenumfang18
ISBN (elektronisch)978-3-031-38854-5
ISBN (Print)9783031388538
DOIs
PublikationsstatusVeröffentlicht - 2023
VeranstaltungProceedings of the 15th International Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2022 - Virtual, Online
Dauer: 9 Feb. 202211 Feb. 2022

Publikationsreihe

NameCommunications in Computer and Information Science
Band1814 CCIS
ISSN (Print)1865-0929
ISSN (elektronisch)1865-0937

Konferenz

KonferenzProceedings of the 15th International Conference on Biomedical Engineering Systems and Technologies, BIOSTEC 2022
OrtVirtual, Online
Zeitraum09.02.202211.02.2022

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