TY - JOUR
T1 - Aminosilane-based functionalization of two-photon polymerized 3D SU-8 microstructures
AU - Aekbote, Badri Lakshmanrao
AU - Jacak, Jaroslaw
AU - Schütz, Gerhard J.
AU - Csányi, Erzsébet
AU - Szegletes, Zsolt
AU - Ormos, Pál
AU - Kelemen, Lóránd
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/10
Y1 - 2012/10
N2 - There is an increasing interest in functionalized complex 3D microstructures with sub-micrometer features for micro- and nanotechnology applications in biology. Depending primarily on the material of the structures various methods exist to create functional layers of simple chemical groups, biological macromolecules or metal nanoparticles. Here an effective coating method is demonstrated and evaluated on SU-8 based 3D microstructures made by two-photon polymerization. Protein streptavidin and gold nanoparticles (NP) were bound to the microstructures utilizing acid treatment-mediated silane chemistry. The protein surface density, quantified with single molecule fluorescence microscopy revealed that the protein forms a third of a monolayer on the two-photon polymerized structures. The surface coverage of the gold NPs on the microstructures was simply controlled with a single parameter. The possible degrading effect of the acid treatment on the sub-micrometer features of the TPP microstructures was analyzed. Our results show that the silane chemistry-based method, used earlier for the functionalization of large-area surfaces can effectively be adapted to coat two-photon polymerized SU-8 microstructures with sub-micrometer features.
AB - There is an increasing interest in functionalized complex 3D microstructures with sub-micrometer features for micro- and nanotechnology applications in biology. Depending primarily on the material of the structures various methods exist to create functional layers of simple chemical groups, biological macromolecules or metal nanoparticles. Here an effective coating method is demonstrated and evaluated on SU-8 based 3D microstructures made by two-photon polymerization. Protein streptavidin and gold nanoparticles (NP) were bound to the microstructures utilizing acid treatment-mediated silane chemistry. The protein surface density, quantified with single molecule fluorescence microscopy revealed that the protein forms a third of a monolayer on the two-photon polymerized structures. The surface coverage of the gold NPs on the microstructures was simply controlled with a single parameter. The possible degrading effect of the acid treatment on the sub-micrometer features of the TPP microstructures was analyzed. Our results show that the silane chemistry-based method, used earlier for the functionalization of large-area surfaces can effectively be adapted to coat two-photon polymerized SU-8 microstructures with sub-micrometer features.
KW - 3D microstructures
KW - Aminosilane treatment
KW - Gold nanoparticle
KW - Optical trap
KW - Surface functionalization
KW - Two-photon polymerization
UR - http://www.scopus.com/inward/record.url?scp=84866061125&partnerID=8YFLogxK
U2 - 10.1016/j.eurpolymj.2012.06.011
DO - 10.1016/j.eurpolymj.2012.06.011
M3 - Article
SN - 0014-3057
VL - 48
SP - 1745
EP - 1754
JO - European Polymer Journal
JF - European Polymer Journal
IS - 10
ER -