Liquefied Natural Gas (LNG) is increasingly used as alternative fuel for heavy-duty vehicles and vessels. However, the specific requirements for handling this cryogenic liquid are sometimes causing uncertainty. To encourage the application of LNG, a deliberate hazard analysis has been carried out in order to determine potential risks and illustrate protective measures. On this basis, required competences for personnel involved in LNG activities have been defined to facilitate safe operations in this industry. Empirical evidence has been gathered by means of online questionnaires and confirmed the relevance of the stated competences. The aim of the study is to raise awareness and knowledge about the safety concerns related to LNG in order to promote its use as an alternative fuel.