TY - GEN
T1 - A versatile micropatterning approach for studying live cell signalling events
AU - Lanzerstorfer, Peter
AU - Müller, Ulrike
AU - Hager, Roland
AU - Dirscherl, Cindy
AU - Gordiyenko, Klavdiya
AU - Niemeyer, Christof M.
AU - Springer, Sebastian
AU - Weghuber, Julian
N1 - Funding Information:
This project was funded by the province of Upper Austria as part of the FH Upper Austria Center of Excellence for Technological Innovation in Medicine (TIMed CENTER) and the Josef Ressel Center for Phytogenic Drug Research financed by the Christian Doppler Forschungsgesellschaft.
Publisher Copyright:
© 2019 CBMS-0001.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2019
Y1 - 2019
N2 - Protein-protein interactions (PPIs) are an important part of biological processes, above all in cellular signal transduction. Here, we present a versatile live cell assay based on antibody micropatterns that allows the visualization, measurement, and quantitation of such interactions in the natural environment of the cell. Methodological variations and the potential of the micropatterning assay are demonstrated on the basis of different biological examples.
AB - Protein-protein interactions (PPIs) are an important part of biological processes, above all in cellular signal transduction. Here, we present a versatile live cell assay based on antibody micropatterns that allows the visualization, measurement, and quantitation of such interactions in the natural environment of the cell. Methodological variations and the potential of the micropatterning assay are demonstrated on the basis of different biological examples.
KW - Micropatterning
KW - Protein-protein interaction
KW - TIRFM
UR - https://www.scopus.com/pages/publications/85094967888
M3 - Conference contribution
AN - SCOPUS:85094967888
T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
SP - 1528
EP - 1529
BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PB - Chemical and Biological Microsystems Society
T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Y2 - 27 October 2019 through 31 October 2019
ER -